Primary isolation medium for Cryptococcus neoformans.
نویسنده
چکیده
The isolation and identification of Cryptococcus neoformans is improved by use of a potato dextrose medium containing urea-antibiotic supplements and a pH of 3.5.
منابع مشابه
Esculin-based medium for isolation and identification of Cryptococcus neoformans.
A simple medium was developed, using esculin as the substrate, for the isolation and identification of Cryptococcus neoformans. C. neoformans produced a brown-black pigment on the medium; all other yeasts produced no pigment or were light yellow. Esculin is beta-glucose-6,7-dihydroxycoumarin. C. neoformans produced pigment because the 6,7-dihydroxycoumarin component of the esculin molecule was ...
متن کاملEvaluation of a simplified Guizotia abyssinica seed medium for differentiation of Cryptococcus neoformans.
A simplified Guizotia abyssinica seed medium, eliminating glucose, creatinine, and phosphate, was evaluated for the isolation and presumptive identification of Cryptococcus neoformans. Of 80 isolates of C. neoformans tested, 69 (86%) developed the characteristic brown pigment within 12 h on this medium as against only 5 isolates (6%) on the complete medium. In primary cultures of experimentally...
متن کاملComparison of Media for the Isolation of Cryptococcus neoformans.
Four media, Staib's Guizotia abyssinica, trypan blue, and Staib's with 2 and 10 mg of methyl violet per liter, were compared for the selective and differential isolation of Cryptococcus neoformans from environmental samples. Trypan blue medium allowed for the differentiation of C. neoformans colonies from Candida albicans colonies several days earlier than did Staib's medium. The addition of me...
متن کاملFirst report of isolation of Cryptococcus neoformans var. neoformans from avian excreta in Kathmandu, Nepal.
This paper delineates the first report on the saprophytic distribution of Cryptococcus neoformans var. neoformans in the city of Kathmandu, Nepal. Twenty-eight samples of old and dry pigeon droppings collected from different sites in Kathmandu were investigated for the presence of C. neoformans by employing a dilution technique. The organism was isolated from seven (25%) of the specimens, repre...
متن کاملRecovery of Cryptococcus neoformans from modified Dubos liquid medium utilized for isolation of mycobacteria.
Cryptococcus neoformans was isolated from nine pathological specimens cultured for mycobacteria. Five of these were recovered only in liquid TBC medium in the absence of growth on conventional substrates.
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ورودعنوان ژورنال:
- Applied microbiology
دوره 18 6 شماره
صفحات -
تاریخ انتشار 1969